Yes, once spent by the new commercial enterprise on its business. Capital invested includes cash contributed to the enterprise and used for its expenses; the requirement is that the full qualifying amount be placed at risk in the enterprise, not held in reserve or returned.
Capital spent by the enterprise is capital at risk
The investor must place the full qualifying amount at risk in the new commercial enterprise for the purpose of generating a return. Money contributed to the restaurant company and then spent on the lease deposit, kitchen equipment, fit-out, licences and opening inventory is plainly at risk, because it is committed to the business and subject to loss. Money held in the investor's personal account against future expenses is not yet invested, and funds contributed but parked in the company's reserve with no business purpose invite the question whether they are actually at risk.
Keep invoices, contracts and bank records showing each expenditure from the enterprise's account. Separately budget the USCIS fees for the investor petition and later Form I-829, listed in the fee schedule, Form G-1055, along with legal, accounting and translation costs; none of those is part of the qualifying investment. If the location's TEA status is the basis for the lower threshold, verify the designation before signing the lease rather than after.